protoscript ii first strand cdna synthesis kit (New England Biolabs)
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protoscript ii first strand cdna synthesis kit
Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pm41832628-78-14-21
Average 99 stars, based on 1853 article reviews
Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdna/ProtoScript+II+First+Strand+cDNA+Synthesis+Kit/pm41832628-78-14-21
Average 99 stars, based on 1853 article reviews
protoscript ii first strand cdna synthesis kit - by Bioz Stars,
2026-10
99/100 stars
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other:Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37 Article Snippet: Oligonucleotides encoding the selected sgRNAs with overhangs compatible with BpiI restriction sites ( ) were annealed in vitro in Annealing buffer (5 mM Tris (#MB01601, NZYtech) pH 7.5–8, 25 mM NaCl (#MB15901, NZYtech) and 0.5 mM EDTA (#20302, VWR) by incubation at 95°C for 5 min, followed by a cool down until 85°C at 2°C/s and from 85°C to 25°C at 0.1°C/s. Article Title: The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37. Article Snippet: Prior to zebrafish microinjection, each plasmid vector was purified with DNA Clean & Concentrator kit (#D4013, ZYMO Research) according to the manufacturer’s recommendations and resuspended in nuclease-free water. Purification:Article Title: NanoTS: a deep learning tool for accurate SNP calling in nanopore long-read transcriptome data. Article Snippet: .. Briefly, 120 ng of Polymerase Chain Reaction:Article Title: CXCR4-tropic HIV-1 infection in an immunocompetent monkey model. Article Snippet: 2.5 mL of blood were collected from naive colony animals in RNA preservation tubes (PAXgene Blood RNA Tube, BD Biosciences #762165) that were processed according to the manufacturer’s instructions. .. Genespecific primers (placed in the 5' and 3' UTRs) were then used to PCR-amplify Article Title: Melatonin Protects Mouse Oocytes From the Environmental Toxicant 6PPD-Quinone by Preserving Mitochondrial and Endomembrane Homeostasis. Article Snippet: N‐(1,3‐dimethylbutyl)‐N′‐phenyl‐p‐phenylenediamine‐quinone (6PPD‐Q) is a ubiquitous tire‐derived transformation product with emerging reproductive toxicity; however, its impact on mammalian oocytes and the potential protective role of melatonin (MT) remain unclear.. Here, we evaluate 6PPD‐Q–induced impairment of mouse oocyte competence and assess the protective efficacy of MT.. 6PPD‐Q exposure significantly decreases polar body extrusion, fertilization, and 2‐cell embryo formation, whereas MT co‐treatment markedly restores these developmental outcomes. Article Title: microRNAs bidirectionally regulate FUT1 to modulate α-1,2-fucosylation and cancer-associated biology Article Snippet: .. FUT1 3’UTR (ENST00000310160.3, 2173 nucleotides) was amplified from Amplification:Article Title: Melatonin Protects Mouse Oocytes From the Environmental Toxicant 6PPD-Quinone by Preserving Mitochondrial and Endomembrane Homeostasis. Article Snippet: N‐(1,3‐dimethylbutyl)‐N′‐phenyl‐p‐phenylenediamine‐quinone (6PPD‐Q) is a ubiquitous tire‐derived transformation product with emerging reproductive toxicity; however, its impact on mammalian oocytes and the potential protective role of melatonin (MT) remain unclear.. Here, we evaluate 6PPD‐Q–induced impairment of mouse oocyte competence and assess the protective efficacy of MT.. 6PPD‐Q exposure significantly decreases polar body extrusion, fertilization, and 2‐cell embryo formation, whereas MT co‐treatment markedly restores these developmental outcomes. Article Title: microRNAs bidirectionally regulate FUT1 to modulate α-1,2-fucosylation and cancer-associated biology Article Snippet: .. FUT1 3’UTR (ENST00000310160.3, 2173 nucleotides) was amplified from Article Title: Flamenco plasticity tunes somatic piRNAs and rewires isoforms, with implications for heritable transposon spread Article Snippet: Reverse transcription was performed with the Transcriptor First Strand cDNA Synthesis Kit (Roche) using oligo(dT) primers. .. The resulting Ligation:Article Title: Melatonin Protects Mouse Oocytes From the Environmental Toxicant 6PPD-Quinone by Preserving Mitochondrial and Endomembrane Homeostasis. Article Snippet: N‐(1,3‐dimethylbutyl)‐N′‐phenyl‐p‐phenylenediamine‐quinone (6PPD‐Q) is a ubiquitous tire‐derived transformation product with emerging reproductive toxicity; however, its impact on mammalian oocytes and the potential protective role of melatonin (MT) remain unclear.. Here, we evaluate 6PPD‐Q–induced impairment of mouse oocyte competence and assess the protective efficacy of MT.. 6PPD‐Q exposure significantly decreases polar body extrusion, fertilization, and 2‐cell embryo formation, whereas MT co‐treatment markedly restores these developmental outcomes. Construct:Article Title: Pervasive enhanced transcription in inflammatory breast cancer tumors and PBMCs impacts RNA splicing and intronic RNAs in plasma Article Snippet: .. TGIRT-seq libraries were constructed as described ( , ) by using TGIRT-template switching from a synthetic RNA template/DNA primer duplex to the 3′ end of the target RNA for 3′ RNA-seq adapter addition and a single-stranded DNA ligation to the 3′ end of the RNA Sequencing:Article Title: Pervasive enhanced transcription in inflammatory breast cancer tumors and PBMCs impacts RNA splicing and intronic RNAs in plasma Article Snippet: .. TGIRT-seq libraries were constructed as described ( , ) by using TGIRT-template switching from a synthetic RNA template/DNA primer duplex to the 3′ end of the target RNA for 3′ RNA-seq adapter addition and a single-stranded DNA ligation to the 3′ end of the DNA Ligation:Article Title: Pervasive enhanced transcription in inflammatory breast cancer tumors and PBMCs impacts RNA splicing and intronic RNAs in plasma Article Snippet: .. TGIRT-seq libraries were constructed as described ( , ) by using TGIRT-template switching from a synthetic RNA template/DNA primer duplex to the 3′ end of the target RNA for 3′ RNA-seq adapter addition and a single-stranded DNA ligation to the 3′ end of the |
